Publications about 'genetic circuits'
Articles in journal or book chapters
  1. T.H. Segall-Shapiro, E. D. Sontag, and C. A. Voigt. Constant gene expression at any copy number using feedforward stabilized promoters. Submitted to Nature Biotechnology, 2017. Keyword(s): synthetic biology, systems biology, genetic circuits, gene copy number.
    This paper deals with the design of promoters that maintain constant levels of expression, whether they are carried at single copy in the genome or on high-copy plasmids. The design is based on an incoherent feedforward loop (iFFL) with a perfectly non-cooperative repression. The circuits are implemented in E. coli using Transcription Activator Like Effectors (TALEs). The resulting stabilized promoters generate near identical expression across different genome locations and plasmid backbones (pSC101, p15a, ColE1, pUC), and also provide robustness to strain mutations and growth media. Further, their strength is tunable and can be used to maintain constant ratios between proteins.



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Last modified: Thu Nov 23 10:40:56 2017
Author: sontag.

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